1.经典细胞培养书Ψ籍下载culture of animal cells（10.7M）

Culture of Animal Cells: A Manual of Basic Technique, 5th Edition
R. Ian Freshney 
ISBN: 978-0-471-45329-1
Hardcover
672 pages
July 2005

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. 


List of Figures. 
List of Color Plates. 

Preface. 

Abbreviations. 

1. Introduction. 

2. Training Programs. 

3. Biology of Cultured Cells. 

4. Laboratory Design and Layout. 

5. Equipment. 

6. Aseptic Technique. 

7. Safety, Bioethics, and Validation. 

8. Culture Vessels and Substrates. 

9. Defined Media and Supplements. 

10. Serum-Free Media. 

11. Preparation and Sterilization. 

12. Primary Culture. 

13. Subculture and Cell Lines. 

14. Cloning and Selection. 

15. Cell Separation. 

16. Characterization. 

17. Differentiation. 

18. Transformation and Immortalization. 

19. Contamination. 

20. Cryopreservation. 

21. Quantitation. 

22. Cytotoxocity. 

23. Culture of Specific Cell Types. 

24. Culture of Tumor Cells. 

25. Organotypic Culture. 

26. Scale-Up. 

27. Specialized Techniques. 

28. Problem Solving. 

29. In Conclusion. 

Appendix I: Preparation of Reagents. 

Appendix II: Sources of Equipment and Materials. 

Appendix III: Suppliers and Other Resources. 

Appendix IV: Glossary. 

Appendix V: General Textbooks and Relevant Journals. 



 

2.酵母遗传学方法实验指南（2.4M）

3.白纯化、免疫、信息学、细胞实验【经典实验汇总（5.4M）

主要内容：

下游蛋白纯化技术电子书：
Native page.exe；SDS-PAGE(SDS—聚丙烯酰胺凝胶电泳「).exe；Western Blot(免疫印迹).exe；包涵体复♂性.exe；超滤.exe；蛋白质浓缩及浓度测定.exe；等电聚焦凝◎胶电泳.exe；多肽人工合成.exe；高效液相.exe；离子＠交换层析.exe；凝胶过滤.exe；亲和层析.exe；细胞破碎.exe；盐析.exe

免疫学电子书：
ELISA.exe；分子诊断.exe；快速诊断.exe；免疫PCR(Immuno PCR,Im-PCR).exe；免疫组化ω　技术.exe；生物素-亲和素系统.exe
疫苗.exe

单抗、多抗及◆其制备电子书籍：
IgG的分离与提※纯.exe；IgY的应︾用与研究进展.exe；单克隆抗体.exe；抗体的纯卐化.exe；抗体介绍.exe；佐剂的作∑　用和制备.exe

生物信息学电子◤书籍：
表达序列标签々（ESTs）及其应用.exe；蛋白质结△构预测.exe；分子进化与系统进化树的构♀建.exe；生物〖信息学概述.exe；生物信←息学数据库及查询.exe；序列【比对及数据库搜索.exe；序列分析预测.exe

真核细胞表达电○子书：
CHO细胞№表达系统.exe；酵母细胞表达√系统.exe；真核细胞表达系↓统.exe

细胞培养及※动物饲养电子书籍
实验动◥物饲养.exe；细胞培养.exe；细胞培养』基.exe

4.蛋■白纯化手册（1.55M）

5.蛋白纯化、信息学、真核细胞表达实验技术大①全

主要内容：
下游蛋白纯化技术电子书
Native page.exe；SDS-PAGE(SDS—聚丙烯酰胺凝胶电泳).exe；Western Blot(免疫印迹).exe；包涵体复性.exe；超滤.exe；蛋白质浓缩及浓度测定.exe；等电聚焦凝胶电泳▆.exe；多肽人工合成.exe；高效液相.exe；离子交换层析.exe；凝胶过滤.exe；亲和层析.exe；细胞破碎.exe；盐析.exe

生物信息学电子书籍：
表达序列标签（ESTs）及其应用.exe；蛋白质结构预测.exe；分子进化与系统进化树的构建.exe；生物信息学概述.exe；生物信息学数据库及查询.exe；序列比对及数据库搜索.exe；序列分析预测.exe

真核细胞表达电子书：
CHO细胞表达系统.exe；酵母细胞表达系统.exe；真核细胞表达系统.exe

细胞▲培养及动物饲养电子书籍
实验动物饲养.exe；细胞培养.exe；细胞培养基.exe

索取方式：请发送邮件到E-Mail到jiahebio@163.com索取，须提供真实▓姓名、所在单位和联系电话，谢谢合作！我们将在3个工作日内将∮您需要的书籍发送到您的手上！